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GI Map Testing

DIAGNOSTIC SOLUTIONS LABORATORY – GI-MAP

The Diagnostic Solutions Laboratory GI Microbial Assay Plus (“GI-MAP”) includes markers for ….

  • gluten sensitivity (anti-gliadin IgA)
  • inflammation in the gut (calprotectin)
  • immunity in the gut (SIgA)
  • pancreatic enzyme sufficiency (elastase)
  • occult blood
  • presence of pathogens – including H Pylori and other bacteria, parasites, worms and viruses – in the gastrointestinal tract.

MARKERS INCLUDED ON THIS TEST:

  •  (Campylobacter, C difficile Toxin A & B, EHEC, E coli 0157, EIEC/Shigella, ETEC LT/ST, Salmonella, Shiga-like Toxins, Shigella, etc.)
  • (Cryptosporidium, Entamoeba histolytica, Giardia)
  • (Adenovirus, Norovirus, Rotavirus)
  • (H Pylori, with 7 virulence factors)
  • (Bacteroides Fragilis, Bifidobacter, Enterococcus, E. coli, Lactobacillus, Clostridium Spp., Enterobacter Spp.)
  •  (Bacteroidetes, Firmicutes, Bateroidetes/Firmicutes ratio)
  • (Bacillus, Enterococcus, Morganella,  Pseudomonas, Staphylococcus, Streptococcus, etc)
  •  (Citrobacter, Klebsiella, Mycobacterium tuberculosis, Prevotella Copri, Proteus Spp etc)
  •  (Blastocystis hominis, Dientamoeba fragilis, Endolimax nana, Entamoeba coli, Chilomastix mesnelli, Cyclospora, Pentatrichomonas)
  •  (Candida albicans, Candida spp., Geotricum spp., Microsporidia spp., Rodotorula spp.)
  •  (Cytomegalovirus, Epstein Barr Virus)
  •  (Ancylostoma duodenale, Ascaris lumbricoides, Necator americanus, Trichuris trichiura, Taenia spp)
  •  (Elastase, Steatocrit, SIgA, Anti-Gliadin, Calprotectin, b-Glucuronidase, Occult Blood)

To detect pathogens, the lab uses Real-time polymerase chain reaction (RT-PCR), or quantitative PCR (qPCR), which combines amplification and detection into one step. qPCR “is one of the most powerful and sensitive gene analysis techniques available to detect the DNA of microorganisms.

TEST DETAILS:

  • Stool (1-day single specimen collection)
  • Nutripath carries the Complete Microbiome Mapping test as an equivalent
  • Additional info:
    • Clients must complete all highlighted sections of the Test Requisition Form and include a copy of the front and back of the insurance card.
    • Do not add diagnostic codes to the form.
    • The lab recommends refrigerating the specimen if it will not be shipped the same or next day.
    • The lab must receive the sample within 6 days of collection (for the SIgA result to be valid). The DNA for the pathogens part of the panel however, is stable for up to 2 weeks.
    • The client does NOT need to be off antimicrobials (antibiotics or herbal supplements) prior to collecting the stool sample (unlike the BH401H which does not allow the use of antimicrobials at least 2 weeks prior to collecting the samples to avoid interference).
    • Orally taken steroids can interfere with the inflammatory markers and if possible the patient should wait 3-4 weeks after the completed regiment to do the GI-MAP. Also, after a colonoscopy, the patient should wait about a week to do the test.
    • The turn-around time to receive the results back is 7-10 business days from when the lab receives the sample

FAQs:

  • Advantages of this test:
    • The GI-MAP is the first FDA-approved DNA/PCR assay for GI pathogens, making the results more widely accepted.
    • PCR technology provides the ability to identify bacteria, parasites, viruses, and fungi regardless of their viability in the sample.
    • Stool cultures grow only aerobic bacteria but 95% of the bacteria in the digestive system are anaerobic, including the pathogens.
    • It is not possible to measure strict anaerobes, viruses, or virulence factors with a culture based test.
    • Parasites need to be in high numbers to be detected by microscopy, so there are times when O&P (Ova & Parasite) testing may miss pathogens that might be detected by PCR.
    • It tests for H pylori and identifies if the H pylori is one of the more virulent strains (cagA or vacA) which has been associated with ulcers and gastric cancer.
    • DNA/PCR assays are superior at detecting Giardia and E histo, as very low amounts of these parasites are typically shed in the stool.
    • DNA/PCR assays are far more sensitive in picking up Candida than culture-based stool tests.
    • In addition to looking for pathogens, this test panel contains several additional markers which can provide invaluable insight into gut immunity (SIgA), gut inflammation (lactoferrin), pancreatic enzyme sufficiency (elastase), and gluten sensitivity (anti-gliadin).

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